Mb-medium.rar Site

: Add the ingredients (excluding agar if making a broth) to 800 ml of distilled water.

: Set the pH to approximately 7.0 using NaOH or HCl. Solidifier : Add the agar if necessary. MB-Medium.rar

: 10.0 g (can vary for Low Salt/High Salt versions) Agar (for solid medium): 15.0 g Preparation Protocol : Add the ingredients (excluding agar if making

: Autoclave the mixture at 121°C (15 psi) for 15 minutes. MB-Medium.rar

: Once cooled to roughly 50-60°C, pour into sterile petri dishes and store in a refrigerator.